Materials and Methods of Morphotaxonomy of Drosophila
For this studyDrosophila were collected from various places of Rajshahi city. The following methods were applied and materials were used.
Total period of specimen’s collection:
The study was made for 5 months. The collection was conducted from March, 2010 to July, 2010.
Specimens were collected for study regularly.
Time and duration of collection:
Specimens were regularly collected in the midday from 10 A.M. to
1 A.M. Sometimes the duration occurred in the afternoon.
Studied Drosophila was collected from fruit and kitchen gardens of Baharampur area, of Rajshahi city.
Equipment used for collection:
The equipment were used during collection:
I. Food trap
II. Plastic bag
III. Hand gloves
IV. Test tube
VIII. Magnifying glass
The equipment were used during preservation:
II. Alcohol 70%,
III. Clear plastic container
The equipment were used in preparing slides:
I. 50, 70, 90, 100% Alcohol.
II. 10% KOH solution.
III. Test tube.
IV. Test tube holder
V. Bunsen burner.
VII. Slides and cover slips.
X. Canada balsam
Regular collections were made from a small fruit and kitchen garden and like other area of Rajshahi city. This was made according to the essential of study.
Collected specimens were preserved in 70% alcohol, or dried. External morphology was observed under a stereoscopic dissecting microscope and metric characters were measured with and steel scale which has draw on millimeter scaling. In the laboratory external morphology was observed under microscope and Drosophila was separated by identifying characters. Males and females were also separated and detailed structures of head, antenna, thorax, abdomen, wings, male and female terminalia were observed.
Specimens were boiled in 10% KOH solution around 1000 C for several minute so that it would be clear to see and observed in a droplet of glycerol under light and compound microscopes.
Total body and parts of the body as antennae, head, thorax, abdomen, wings and genitalia were observed. Photomicrographs were taken.
Separated parts of the body of Drosophila which were clear to see after boiling in 10% KOH, were kept on a slide. The procedure for whole mount preparation is given below:
a) 70% alcohol used for 3-5 minutes on the parts of the body.
b) 100% alcohol used for 5-10 minutes on the parts of the body.
c) Xylene used for 3-5 minutes on the parts of the body.
d) Covered with cover slips by Canada balsam.